Coding
Part:BBa_K3930003:Design
Designed by: Thomas Gaudin Group: iGEM21_Toulouse_INSA-UPS (2021-10-07)
α-ionone induction system and expression in Saccharomyces cerevisiae (pViolette)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 5805
Illegal PstI site found at 5798 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 5805
Illegal PstI site found at 5798 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1265
Illegal BglII site found at 1795
Illegal BamHI site found at 1379
Illegal BamHI site found at 3518
Illegal BamHI site found at 4370
Illegal XhoI site found at 73 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 5805
Illegal PstI site found at 5798 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 5805
Illegal PstI site found at 5798
Illegal NgoMIV site found at 5303
Illegal AgeI site found at 606
Illegal AgeI site found at 5021 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4761
Design Notes
According to Takara kit handbook advice and our experiments, a GC-rich homology zone is less likely to work for inFusion cloning. We therefore advise future iGEM teams to have homology zones for InFusion that flank such sequences
Source
NsrR, LcyE and ofCCD1 fragments were PCR amplified from IDT or Twist Bioscience gblocks and pCfB3040 XII-4 was linearized by inverse PCR.